Journal: bioRxiv
Article Title: Modeling herpes simplex virus type 2 and Zika virus replication in vaginal organoids and spheroids
doi: 10.64898/2026.03.02.709097
Figure Lengend Snippet: NS1 concentration in the supernatant (A) and NS5 transcript expression (B) in mouse vaginal organoids infected with ZIKV at MOI of 1. Values normalized to uninfected samples (Ni) and reported as fold change. (C) NS4B staining in organoids sections from 48 hours post-infection. ZIKV titer of mouse vaginal organoids (D) and VK2 spheroids (E) infected with ZIKV in the presence of 10 µM RDV. Values are mean ± SEM of 3 biological replicates. One-way ANOVA or two-way ANOVA with Tukey’s test for multiple comparisons was performed for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Magnification 63X; scale bars represent 50 µm. LD, limit of detection (5 PFU/ml). h, hours post-infection.
Article Snippet: ZIKV titer from infection experiments was quantified using an ELISA to detect NS1 concentration (Sino Biological, Cat No. KIT40544).
Techniques: Concentration Assay, Expressing, Infection, Staining